Epigenome Editing

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An Epigenome Editing is a genome engineering task in which the epigenome is modified at specific sites using engineered molecules targeted to those sites (as opposed to whole-genome modifications).



References

2022

  • (Wikipedia, 2022) ⇒ https://en.wikipedia.org/wiki/Epigenome_editing Retrieved:2022-1-7.
    • Epigenome editing or Epigenome engineering is a type of genetic engineering in which the epigenome is modified at specific sites using engineered molecules targeted to those sites (as opposed to whole-genome modifications). Whereas gene editing involves changing the actual DNA sequence itself, epigenetic editing involves modifying and presenting DNA sequences to proteins and other DNA binding factors that influence DNA function. By "editing” epigenomic features in this manner, researchers can determine the exact biological role of an epigenetic modification at the site in question.

      The engineered proteins used for epigenome editing are composed of a DNA binding domain that target specific sequences and an effector domain that modifies epigenomic features. Currently, three major groups of DNA binding proteins have been predominantly used for epigenome editing: Zinc finger proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR).